Chimeric receptors for the generation of...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/12 (2006.01) A61K 35/14 (2006.01) C07K 14/705 (2006.01) C07K 14/725 (2006.01) C12N 5/06 (2006.01) C12N 5/10 (2006.01) C12N 15/62 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2204183

The invention provides cytotoxic T lymphocytes that express a chimeric cell surface receptor. The chimeric receptor enables the lymphocytes to become activated and to proliferate (via a TH-independent autocrine cycle) in response to adhesion between the lymphocyte and an antigen displaying cell (ADC) that might not normally activate the T cell. The chimeric receptors of the present invention comprise fusion polypeptides of the following structure: XC-TM-IC28, wherein (XC) is an extracellular region derived from a receptor for a ligand that is expressed on the surface of an antigen-displaying cell, fused via a transmembrane region (TM) to an intracellular region (IC28) which is derived from the intracellular region of the CD28 receptor.

L'invention porte sur des lymphocytes T cytotoxiques exprimant un récepteur chimère de surface cellulaire. Ledit récepteur chimère permet l'activation et la prolifération des lymphocytes (par l'intermédiaire d'un cycle autocrine indépendant des cellules T¿H?) en réponse à l'adhésion entre le lymphocyte et une cellule présentant un antigène (ADC) qui pourrait ne pas activer la cellule T en temps normal. Les récepteurs chimères de la présente invention comportent des polypeptides de fusion de structure XC-TM-IC28 dans laquelle (XC) est une région extracellulaire dérivée du récepteur d'un ligand exprimé à la surface d'une cellule présentant un antigène, fusionnée par l'intermédiaire d'une région transmembranaire (TM) une région intracellulaire (IC28) dérivée de la région intracellulaire du récepteur CD28.

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