Chimeric regulatory regions and gene cassettes for...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/82 (2006.01) A01H 5/00 (2006.01)

Patent

CA 2174954

Chimeric regulatory regions and gene cassettes based upon Agrobacterium tumefaciens opine synthase genes are provided for expressing foreign genes in plants. Various upstream activating sequences from opine synthase genes like the mannopine synthase and the octopine synthase genes are operably linked with protomers (or protomers plus activating sequences), both of which are then operably linked to the foreign gene. These regions and cassettes permit expression levels and patterns that could not be obtained previously.

L'invention concerne des régions régulatrices chimères ainsi que des cassettes de gènes à base de gènes d'opine synthase d'Agrobacterium tumefaciens, destinées à l'expression de gènes étrangers dans des plantes. On lie fonctionnellement diverses séquences d'activation amont de gènes d'opine synthase tels que des gènes de mannopine synthase et d'octopine synthase, à des promoteurs (ou promoteurs plus séquences d'activation), puis on les lie fonctionnellement aux gènes étrangers. Ces régions et cassettes permettent d'obtenir des niveaux et des configurations d'expression que l'on ne pouvait obtenir auparavant.

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