Chiral synthesis with modified enzymes

Details Chiral synthesis with modified enzymes Chiral synthesis with modified enzymes

C - Chemistry, Metallurgy

12

N

C12N 15/52 (2006.01) C12N 9/00 (2006.01) C12N 9/04 (2006.01) C12P 7/42 (2006.01)

Patent

CA 2127126

2127126 9315208 PCTABS00024 A method for modifying the specificity and/or efficiency of an enzyme, while retaining its catalytic activity, characterised in that it comprises: selecting an enzyme, the tertiary structure of which is substantially known or deduced; identifying at least one specificity and/or efficiency-related region; identifying or constructing unique restriction sites bounding the identified region in the DNA coding therefor; generating a DNA sequence which corresponds to at least a portion of the identified region, except that the nucleotides of at least one codon are randomized, or selecting as a substitute for at least a portion of the identified region an alternative such region, which may itself be similarly randomized; using the generated or substitute DNA sequence to replace the original such sequence; expressing the DNA including the generated or substitute DNA sequence; and selecting for a desired modification so that the DNA coding therefor may be isolated is disclosed.

Affiliated with

Also associated with

Rating

Chiral synthesis with modified enzymes does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Chiral synthesis with modified enzymes, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Chiral synthesis with modified enzymes will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFCA-PAI-O-1701352