C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/56 (2006.01) A01H 5/00 (2006.01) C12N 1/21 (2006.01) C12N 5/10 (2006.01) C12N 9/24 (2006.01) C12N 15/82 (2006.01) C12N 15/84 (2006.01) A01N 65/00 (2006.01)
Patent
CA 2169833
The present invention provides a novel class of plant chitinases, which have endo-chitinase activity, antifungal activity and a molecular weight of about 40 to 43 kDa as judged by Sodium Dodecyl Sulphate Polyacrylamide electrophoreses. The invention further provides antifungal compositions comprising as active ingredient a chitinase according to the invention. According to a different aspect of the invention an isolated DNA sequence is provided which comprises an open reading frame encoding a novel chitinase. The invention also encompasses a plant expressible recombinant DNA sequence which comprises a DNA sequence encoding a chitinase according to the invention, as well as plant cells and plants which have such recombinant DNA incorporated into their genome.
La présente invention se rapporte à une nouvelle classe de chitinases végétales à activité d'endo-chitinase, à activité antifongique et d'un poids moléculaire compris entre 40 46 43 kDa tel qu'évalué par électrophorèse sur polyacrylamide de sulfate de dodécyle de sodium. L'invention se rapporte en outre à des compositions antifongiques comprenant, comme principe actif, une chitinase selon l'invention. Selon un autre aspect de l'invention, une séquence d'ADN isolée est décrite, laquelle comprend un cadre ouvert de lecture codant une nouvelle chitinase. L'invention se rapporte également à une séquence d'ADN recombinant pouvant être exprimé dans les plantes, qui comprend une séquence d'ADN codant une chitinase selon l'invention, ainsi qu'à des cellules végétales et à des plantes comprenant un tel ADN recombinant incorporé dans leur génome.
Apotheker-De Groot Marion
Bol John Ferdinand
Clemens-Cornelissen Bernardus Johannes
Linthorst Hubertus Josephus Maria
Melchers Leo Sjoerd
Osler Hoskin & Harcourt Llp
Rijksuniversiteit Te Leiden
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