Chitosan-derivatives for gene delivery and expression

A - Human Necessities – 61 – K

Patent

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Details

A61K 47/36 (2006.01) A61K 9/00 (2006.01) A61K 9/127 (2006.01) A61K 9/51 (2006.01)

Patent

CA 2516188

The present invention provides particles comprising chitosan, or a derivative thereof, useful as delivery vehicles for polynucleotides encoding polypeptides, compositions comprising such particles and a pharmaceutically acceptable carrier, and methods for delivering polynucleotides using such particles. Optionally, the particles of the invention further comprise a lipid component. The present further provides a method for enhancing interferon- gamma expression to regulate the production of cytokines secreted by T-helper type 2 (Th2) cells within a subject by administering an effective amount of a particle of the subject invention to the subject, wherein the particle comprises a polynucleotide encoding interferon-gamma.

La présente invention concerne des particules comprenant du chitosane, ou un dérivé de ce composé, ces particules étant utiles en tant que véhicules de transfert pour des polynucléotides codant pour des polypeptides. L'invention concerne également des compositions comprenant lesdites particules et un support pharmaceutiquement acceptable, ainsi que des procédés de transfert de polynucléotides au moyen de ces particules. Eventuellement, les particules selon l'invention peuvent comprendre en outre un composant lipidique. L'invention concerne par ailleurs un procédé permettant d'améliorer l'expression de l'interféron gamma pour réguler la production de cytokines sécrétées par les lymphocytes T auxiliaires de type 2 (Th2) chez un sujet par administration audit sujet d'une dose efficace d'une particule selon l'invention, ladite particule comprenant un polynucléotide codant pour l'interféron gamma.

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