Chlamydia half-sandwich immunoassay

Details Chlamydia half-sandwich immunoassay Chlamydia half-sandwich immunoassay

G - Physics

01

N

167/45

G01N 33/535 (2006.01) G01N 33/537 (2006.01) G01N 33/569 (2006.01)

Patent

CA 2035646

ABSTRACT OF THE DISCLOSURE A method for detecting the presence of Chlamydia trachomatis in a test sample, such as a clinical specimen containing infected human cells, in which the test sample is treated to expose multivalent Chlamydia antigens from C. trachomatis present in the sample and a labeled antibody reagent is added to form a mixture comprising immune complexes of the labeled antibody and released antigens. The mixture is passed through a glass fiber depth filter that is impervious to the resulting immune complexes and the label that is retained by the filter is detected. The half-sandwich method is sensitive and rapid, and requires a minimum number of reagents and assay steps.

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