C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/56 (2006.01) C07H 21/00 (2006.01) C12N 5/10 (2006.01) C12N 9/24 (2006.01) C12N 15/01 (2006.01) C12P 21/00 (2006.01) C12Q 1/68 (2006.01) A61K 38/00 (2006.01) C12N 5/06 (2006.01)
Patent
CA 2160458
A recombinant cell line has a constitutive sialidase whose functional expression is disrupted, for example by homologous recombinant or using antisense RNA. Sialidase is purified from cell culture fluid of Chinese hamster ovary cells. DNA encoding sialidase is obtained using an oligonucleotide probe designed using amino acid sequence data on the sialidase, and the DNA is expressed in host cells transformed with the DNA.
Sliwkowski Mary B.
Warner Thomas G.
Dennison Associates
Genentech Inc.
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