C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
195/1.14, 195/1.
C12N 15/53 (2006.01) C07K 16/40 (2006.01) C12N 1/21 (2006.01) C12N 5/16 (2006.01) C12N 9/08 (2006.01)
Patent
CA 2024562
There are given disclosures on a method of measuring an antimicrosomal antibody, a gene engineering process for preparing human thyroid peroxidase to be used for the measurement as well as an expression of the human thyroid peroxidase. The antimicrosomal antibody is measured by checking and investigating a reactivity of the human thyroid peroxidase produced by gene engineering techniques with a human serum. A natural membrane bound type human thyroid peroxidase or a secretion type human thyroid peroxidase is produced. The natural membrane bound type human peroxidase is produced on cell membrane of an cultivated animal cell or Escherichia coli transformed with an expression vector having therein recombinant DNAs encoding the human thyroid gene and dihydrofolate reductase gene. The secretion type human thyroid peroxidase is produced in a supernatant of cultivated medium of animal cell transformed with an expression vector having therein recombinant DNAs encoding the human thyroid gene but with no membrane region thereof and dihydrofolate reductase gene.
Hata Junichiro
Kabeno Shouko
Kato Hideo
Matsumoto Ken
Yagihashi Satoru
Hata Junichiro
Kabeno Shouko
Kato Hideo
Marks & Clerk
Matsumoto Ken
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