Cholesterol esterases with variable substrate specificity

Details Cholesterol esterases with variable substrate specificity Cholesterol esterases with variable substrate specificity

C - Chemistry, Metallurgy

12

N

C12N 15/55 (2006.01) C12N 9/18 (2006.01) C12Q 1/60 (2006.01)

Patent

CA 2064202

2064202 9118987 PCTABS00008 Cholesterol esterases with variable substrate specificity are made by cloning of a cholesterol esterase gene whose active centre contains the sequence -Gly-His-Ser-X-Gly, where X stands for an amino acid, in a vector, transformation of a microorganism with this vector and expression of the cholesterol esterase gene. The amino acid X of the active centre is interchanged with another amino acid by mutagenesis.

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