Choline oxidase and method for producing the same

Details Choline oxidase and method for producing the same Choline oxidase and method for producing the same

C - Chemistry, Metallurgy

12

N

195/123, 195/39,

C12N 9/04 (2006.01) C12N 1/20 (2006.01)

Patent

CA 1324586

ABSTRACT OF THE DISCLOSURE A novel choline oxidase being thermostable at 50°C at pH 7 to 9 is disclosed. A method for producing the enzyme by using actinomycetes is also disclosed. The production method comprises culturing an actinomycete in a culture medium and recovering the choline oxidase, the actinomycete being capable of producing chlorine oxidase and of growing at a temperature not lower than 45°C. The novel choline oxidase has the following properties: (a) optimal pH within the range of 7.5 to 9; (b) stable pH range of 7 to 9; (c) isoelectric point within the range of 4.5 to 5.5; (d) molecular weight of 50,000 to 95,000 (determined by gel-filtration method) or 5.5 x 104 to 6.5 to 104 (determined by SDS-polyacrylamide electrophoresis); and (e) optimal temperature within the range of 40 to 55°C. The choline oxidase may be used in chemical analyses and clinical tests.

609430

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