Chondroitin synthase

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/09 (2006.01) C12N 5/10 (2006.01) C12N 9/10 (2006.01) C12P 19/18 (2006.01) C12P 19/26 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2456120

A vector containing DNA encoding a protein having the amino acid sequence represented by amino acid numbers 47 to 802 in SEQ ID NO:2 or its equivalent. By expressing this DNA, human chondroitin synthase can be obtained. Using this human chondroitin synthase, a sugar chain having a disaccharide repetitive unit of chondroitin can be produced. The above-described DNA or a fragment thereof is usable as a hybridization probe for the human chondroitin synthase.

L'invention concerne un vecteur contenant l'ADN codant pour une protéine ayant la séquence d'acides aminés représentée par les numéros d'acides aminés 47 à 802 de SEQ ID NO :2 ou ses équivalents. L'expression de cet ADN permet d'obtenir la synthase de chondroitine humaine. Cette synthase de chondroitine humaine permet de produire une chaîne de glucides ayant une unité répétitive disaccharidique de chondroitine. On peut utiliser l'ADN susmentionné ou un fragment de ce dernier comme échantillon d'hybridation pour la synthase de chondroitine.

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