Chondroitinase, process for preparing the same, and...

C - Chemistry – Metallurgy – 12 – N

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C12N 9/88 (2006.01) A61K 38/51 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2099138

A crystallizable, purified chondroitinase ABC having a molecular weight of about 100,000 dalton by the measurement of the SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and the measurement by the gel permeation chromatography method, having alanine as the N-terminal amino acid and proline as the C-terminal amino acid. A process for the purification of the crystallizable purified chondroitinase ABC comprising removing nucleic acid from an surfactant solution extract obtained from cells of chondroitinase ABC-producing microorganisms and chromatographically treating by concentration gradient elution using a weak cation exchange resin or a strong cation exchange resin. A composition comprising a chondroitinase and serum albumin, gelatin, or a nonionic surfactant.

Chondroïtinase ABC purifiée, cristallisable, possédant une masse molaire d'environ 100 000 u.m.a., déterminée par électrophorèse sur gel de SDS-polyacrylamide (SDS-PAGE), et par chromatographie avec perméation de gel, l'alanine étant l'acide aminé sur le N terminal et la proline l'acide aminé sur le C terminal. Une méthode de purification de la chondroïtinase ABC cristallisable consiste à éliminer un acide nucléique d'un extrait de solution de surfactif, provenant de cellules de microorganismes productrices de chondroïtinase ABC et à appliquer un traitement par chromatographie avec élution par gradient de concentration, à l'aide d'une résine échangeuse de cation fort, ou d'une résine échangeuse de cation faible. Composition renfermant une chondroïtinase et de l'albumine sérique, de la gélatine, ou un surfactif non ionique.

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