C - Chemistry – Metallurgy – 07 – K
Patent
C - Chemistry, Metallurgy
07
K
C07K 1/18 (2006.01) B01D 15/08 (2006.01) C07K 1/14 (2006.01) C07K 1/16 (2006.01) C07K 16/00 (2006.01) C07K 16/06 (2006.01) G01N 30/38 (2006.01) G01N 30/60 (2006.01)
Patent
CA 2047902
21 CHROMATOGRAPHY CARTRIDGE ABSTRACT OF THE DISCLOSURE A chromatographic method and apparatus for purifying proteins generally, and monoclonal antibodies from ascites fluids specifically, achieves results superior to other methods. The method comprises contacting a solution containing proteins to be purified with a porous, substantially nonswellable sheet which has hydrated crystalline hydroxylapatite particles physically immobilized therein, in the presence of a pH buffer, and then eluting purified protein fractions, such that the flow rate and back pressure do not change significantly throughout the method. One preferred apparatus comprises inlet and outlet seals, spiral-wound or core-wrapped chromatographic separation medium comprising hydrated crystalline hydroxylapatite particles physically immobilized in a porous, substantially nonswellable sheet, and a porous central support upon which the sheet is wrapped. Another apparatus comprises single or stacked chromatographic separation medium comprising hydrated crystalline hydroxylapatite particles physically immobilized in a porous, substantially nonswellable sheet. PS\DOCS\5084
Cummings Larry J.
Taylor Michael A.
Bio-Rad Laboratories Inc.
Fetherstonhaugh & Co.
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