Chromatography columns with continuous beds formed in situ...

G - Physics – 01 – N

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G01N 30/60 (2006.01) B01D 15/08 (2006.01) G01N 30/56 (2006.01)

Patent

CA 2225703

A chromatography column with a continuous solid bed spanning the cross section of the column, the bed containing channels large enough for hydrodynamic flow, is prepared by polymerizing a mixture of monomers and ammonium sulfate in an aqueous solution. The monomers include a monofunctional monomer such as a vinyl, allyl, acrylic, or methacrylic compound, and a polyfunctional monomer (i.e., a crosslinker), the total monomer concentration being in the range of 10% to 20% by weight, the mole ratio of crosslinker to total monomer being in the range of 0.3 to 0.4, and the ammonium sulfate having a concentration in the range of 0.4 M to 0.8 M. Functional groups to impart specialized separation capabilities, notably anion and cation exchange, can be included in the monomer mixture.

Une colonne de chromatographie caractérisée par un lit solide continu traversant la section transversale de la colonne, le lit contenant des canaux de taille suffisante pour permettre un écoulement hydrodynamique, est préparée par polymérisation d'un mélange de monomères et de sulfate d'ammonium dans une solution aqueuse. Les monomères comprennent un monomère monofonctionnel comme un composé vinylique, allylique, acrylique ou méthacrylique ainsi qu'un monomère polyfonctionnel (c.-à-d., un agent de réticulation); la concentration totale de monomère varie de 10 % à 20 % en poids, le rapport molaire de l'agent de réticulation au monomère total se situe dans une étendue de 0,3 à 0,4 et la concentration de sulfate d'ammonium varie de 0,4 M à 0,8 M. Des groupes fonctionnels destinés à conférer des caractéristiques de séparation spéciales, notamment pour l'échange anionique et cationique, peuvent être ajoutés dans le mélange monomérique.

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