B - Operations – Transporting – 01 – D
Patent
B - Operations, Transporting
01
D
B01D 15/38 (2006.01) C07K 1/22 (2006.01) C07K 14/31 (2006.01) G01N 33/53 (2006.01)
Patent
CA 2664530
The present invention relates to a chromatography ligand, which comprises Domain C from Staphylococcus protein A (SpA), or a functional fragment or variant thereof. The chromatography ligand presents an advantageous capability of withstanding harsh cleaning in place (CIP) conditions, and is capable of binding Fab fragments of antibodies. The ligand may be provided with a terminal coupling group, such as arginine or cysteine, to facilitate its coupling to an insoluble carrier such as beads or a membrane. The invention also relates to a process of using the ligand in isolation of antibodies, and to a purification protocol which may include washing steps and/or re-generation with alkali.
La présente invention concerne un ligand de chromatographie comprenant un domaine C issu de la protéine A de Staphylococcus (SpA), ou un fragment ou un variant fonctionnel correspondant. Ce ligand de chromatographie présente la capacité avantageuse de résister à des conditions de nettoyage en place (NEP) agressives, et peut se lier aux fragments d'anticorps Fab. Ledit ligand peut comprendre un groupe de couplage terminal, tel qu'une arginine ou une cystéine, facilitant son couplage avec un support insoluble tel que des billes ou une membrane. L'invention concerne également un procédé d'utilisation de ce ligand pour l'isolement d'anticorps, ainsi qu'un protocole de purification pouvant comprendre des opérations de lavage et/ou une régénération avec un alcali.
Aberg Per-Mikael
Hall Martin
Larsson Sture
Muranyi Andreas
Rodrigo Gustav
Fetherstonhaugh & Co.
Ge Healthcare Bio-Sciences Ab
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