C - Chemistry – Metallurgy – 07 – H
Patent
C - Chemistry, Metallurgy
07
H
260/210, 260/229
C07H 19/04 (2006.01) C07H 21/00 (2006.01) C07H 23/00 (2006.01)
Patent
CA 1194861
ABSTRACT Novel substrates for monitoring of catalytic activity resulting in hydrolytic release of products which can be readily detected by spectrophotometric or fluorometric means are provided. The substrates have the general formula: Image wherein B is a nucleotide base capable of assisting in hydro- lysis of the phosphate ester at the 3'-position; R is a moiety selected from the group consisting of umbelliferonyl, 4-methyl- umbelliferonyl, 3-flavonyl, o-nitrophenyl, m-nitrophenyl, p-nitrophenyl, dinitrophenyl, cyanophenyl, acylphenyl, carboxy- phenyl, phenylsulfonate, phenylsulfonyl and phenylsulfoxide; R' is a moiety selected from the group consisting of hydrogen, alkyl, alkenyl cycloalkyl, aryl, araalkyl, acyl, oxaalkyl, thioalkyl, oxacycloalkyl, and thiocycloalkyl and R" is hydro- gen or a cation selected from the group consisting of calcium, barium, lithium, sodium, ammonium, substituted ammonium and pyridinium. The substrates are capable of undergoing cata- lytic-induced hydrolysis of the phosphate ester at the 3'- position to yield a species capable of beinq monitored spectro- photometrically or fluorometrically.
397669
Gohlke James R.
Hedaya Eddie
Kang Jemo
Mier Jeanette D.
Osler Hoskin & Harcourt Llp
Roche Diagnostics Corporation
LandOfFree
Chromogenic and/or fluorogenic substrates for monitoring... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Chromogenic and/or fluorogenic substrates for monitoring..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Chromogenic and/or fluorogenic substrates for monitoring... will most certainly appreciate the feedback.
Profile ID: LFCA-PAI-O-1278269