Chromogenic dibenzoxazepinone enzyme substrates

Details Chromogenic dibenzoxazepinone enzyme substrates Chromogenic dibenzoxazepinone enzyme substrates

C - Chemistry, Metallurgy

07

D

150/15, 260/228,

C07D 267/18 (2006.01) C07D 281/14 (2006.01) C07F 9/6527 (2006.01) C07F 9/6536 (2006.01) C07H 15/26 (2006.01) C07H 17/00 (2006.01) C12Q 1/34 (2006.01) C12Q 1/40 (2006.01)

Patent

CA 2013525

Chromogenic enzyme substrate compounds comprising a dibenz [b,e][1,4]oxazepinone or dibenzo[b,e][1,4]thiazepinone nucleus having an enzyme-cleavable group such as a radical of a sugar, carboxylic acid, amino acid, peptide, phosphoric acid, or sulfuric acid. The substrate compounds are, in general, highly soluble in aqueous media and only slightly colored, and produce, upon enzyme cleavage, a chromogen exhibiting a large change in absorbance and a pKa below 7. Such substrates find use as indicators for the determination of enzyme analytes and enzymes used as markers in a variety of assays, including immunoassays.

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