C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
150/15.2, 150/3.
C12Q 1/34 (2006.01) C09B 43/18 (2006.01) C12Q 1/42 (2006.01) C12Q 1/44 (2006.01) C12Q 1/46 (2006.01)
Patent
CA 1332806
1. Chromogenic substrates for the detection of hydro- lyzing enzymes, processes for the preparation of these chromogenic substrates and the use of the chromogenic substrates. 2.1 The quantification of hydrolyzing enzymes for diagnostic purposes has to date been carried out by determination of the amounts of a fluorescent or highly absorbent chromogenic dyestuff liberated in the hydrolytic reaction, detection of which has in part been possible exclusively by instruments or in the neutral to alkaline pH range. After enzymatic hydrolysis, the chromogenic substrates according to the invention lead to highly sensitive and specifically measurable signals regardless of the pH range. 2.2 Azo dyestuff compounds with the general formula A - N = N - B (OR) in which A) and B) have the meanings given in claim 1 and R) is a radical which can be liberated by enzymatic hydrolysis, excluding a carbonyl radical, are provided. After enzymatic hydrolysis, highly sensitively and specifically measurable signals are obtained regardless of the pH range. 2.3 The azo dyestuff compounds mentioned are used for the detection of hydrolyzing enzymes, in particular glycosidases, phosphatases and sulfatases.
578824
Bereskin & Parr Llp/s.e.n.c.r.l.,s.r.l.
Dade Behring Marburg Gmbh
LandOfFree
Chromogenic substrates does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Chromogenic substrates, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Chromogenic substrates will most certainly appreciate the feedback.
Profile ID: LFCA-PAI-O-1205498