Chromosomal saturation mutagenesis

Details Chromosomal saturation mutagenesis Chromosomal saturation mutagenesis

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C12N 1/15 (2006.01) C12N 1/19 (2006.01) C12N 1/21 (2006.01) C12N 5/10 (2006.01) C12N 15/09 (2006.01)

Patent

CA 2468710

In One aspect, the invention provides methods for Chromosomal Saturation Mutagenesis (CSM) comprising generating randomly mutated, overlapping segments for an entire chromosome using error-prone PCR or other techniques, and libraries of nucleic acids made by these methods.

Selon une réalisation, l'invention porte sur des procédés s'appliquant à la mutagenèse à saturation chromosomique et consistant à générer des segments chevauchants à mutation aléatoire d'un chromosome entier par PCR sujette à l'erreur ou autres techniques. L'invention porte également sur des bibliothèques d'acides nucléiques obtenues par ces procédés.

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