Chromosome-wide analysis of protein-dna interactions

Details Chromosome-wide analysis of protein-dna interactions Chromosome-wide analysis of protein-dna interactions

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2383928

The present invention relates to a method of identifying a region (one or more) of a genome of a cell to which a protein of interest binds. In the methods decribed herein, DNA binding protein of a cell is linked (e.g. covalently crosslinked) to genomic DNA of a cell. The genomic DNA to which the DNA binding protein is linked is removed and combined or contacted with DNA comprising a sequence complementary to genomic DNA of the cell under conditions in which hybridization between the identified genomic DNA and the sequence complementary to genomic DNA occurs. Region(s) of hybridization are region(s) of the genome of the cell to which the protein binds.

La présente invention porte sur un procédé d'identification d'une région (ou de plusieurs régions) d'un génome d'une cellule auquel est liée une protéine. Selon les procédés décrits, la protéine de liaison à l'ADN d'une cellule est liée (c.-à-d. réticulée de manière covalente) à l'ADN génomique d'une cellule. L'ADN génomique auquel la protéine de liaison est liée est retiré et combiné ou mis en contact avec un ADN comprenant une séquence complémentaire à l'ADN génomique de la cellule dans des conditions où se produit l'hybridation entre l'ADN génomique identifié et la séquence complémentaire à l'ADN génomique. La ou les régions d'hybridation sont les régions du génome de la cellule auquel se lie la protéine.

Affiliated with

Also associated with

Rating

Chromosome-wide analysis of protein-dna interactions does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Chromosome-wide analysis of protein-dna interactions, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Chromosome-wide analysis of protein-dna interactions will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFCA-PAI-O-1702508