C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01)
Patent
CA 2439477
The present invention provides methods and kits for the rapid exponential amplification of nucleic acid molecules using a padlock probe. The present invention improves upon the existing methods for amplifying padlock probes by eliminating or delaying the appearance of artifact products that cause false positive results, and also increase the sensitivity and speed of the assay. Further provided are nucleic acid amplification primers containing non- informative base analogs.
La présente invention traite de procédés et de kits pour assurer l'amplification exponentielle rapide de molécules d'acide nucléique à l'aide d'une sonde de verrouillage. La présente invention permet d'améliorer les procédés actuels pour amplifier les sondes de verrouillage en éliminant ou retardant l'aspect de produits d'artéfacts qui donnent des résultats positifs faux, et augmentent également la sensibilité et la vitesse du dosage. En outre, l'invention concerne des amorces d'amplification d'acide nucléique contenant des analogues de base non informatifs.
Gowling Lafleur Henderson Llp
Virco Bvba
LandOfFree
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