Circulating mrna as diagnostic markers for pregnancy-related...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01)

Patent

CA 2513292

Methods and kits are provided for diagnosing, monitoring, or predicting the conditions of pre-eclaimpsia, fetal chromosomal aneuploidy, and pre-term labor in a pregnant woman, as well as for detecting pregnancy in a woman, by quantitatively measuring in the maternal blood the amount of one or more mRNA species encoding human chorionic gonadotropin .beta. subunit (hCG-.beta.), human placental lactogen (hPL), human corticotropin releasing hormone (hCRH), KiSS-1 metastasis-suppressor (KISS1), tissue factor pathway inhibitor 2 (TPFI2), placenta-specific 1 (PLAC1), or glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and comparing the amount of the Methods and kits are provided for diagnosing, monitoring, or predicting the conditions of pre- eclaimpsia, fetal chromosomal aneuploidy, and pre-term labor in a pregnant woman, as well as for detecting pregnancy in a woman, by quantitatively measuring in the maternal blood the amount of one or more mRNA species encoding human chorionic gonadotropin .beta. subunit (hCG-.beta.), human placental lactogen (hPL), human corticotropin releasing hormone (hCRH), KiSS-1 metastasis-suppressor (KISS1), tissue factor pathway inhibitor 2 (TPFI2), placenta-specific 1 (PLAC1), or glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and comparing the amount of the mRNA species with a standard control.

L'invention concerne des méthodes et des nécessaires pour le diagnostic, le monitorage ou la prévision d'états de pré-éclampsie, d'aneuploïdie chez le foetus, et d'accouchement prématuré chez une femme enceinte, et pour la détection d'une grossesse chez une femme, consistant à mesurer quantitativement dans le sang maternel la concentration d'une ou plusieurs espèces d'ARNm codant pour une sous-unité de la .beta.-gonadotrophine chorionique humaine (hCG-.beta.), le lactogène placentaire humain (hPL), l'hormone de libération de la corticotrophine humaine (hCRH), le suppresseur de métastases Kiss-1 (KISS1), l'inhibiteur de la voie du facteur tissulaire 2 (TPFI2), le gène d'expression spécifique du placenta 1 (PLAC1), ou la glycéraldéhyde-3-phosphate déshydrogénase (GAPDH), et à comparer la concentration des espèces d'ARNm à une valeur de contrôle.

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