Classification of nucleic acid templates

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) C12N 15/10 (2006.01)

Patent

CA 2746632

Methods, compositions, and systems are provided for characterization of modified nucleic acids. In certain preferred embodiments, single molecule sequencing methods are provided for identification of modified nucleotides within nucleic acid sequences. Modifications detectable by the methods provided herein include chemically modified bases, enzymatically modified bases, abasic sites, non-natural bases, secondary structures, and agents bound to a template nucleic acid.

La présente invention concerne des procédés, compositions, et systèmes, destinés à la caractérisation d'acides nucléiques modifiés. Certains modes de réalisation préférés concernent des procédés de séquençage de molécules uniques permettant d'identifier des nucléotides modifiés dans les séquences d'acides nucléiques. Les modifications détectables grâce aux procédés de l'invention concernent les bases chimiquement modifiées, les bases modifiées par voie enzymatique, les sites dépourvus de bases, les bases non-naturelles, les structures secondaires, et les agents liés à un acide nucléique de matrice.

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