Clean genome bactofection

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/85 (2006.01) C12N 15/70 (2006.01) C12N 15/87 (2006.01)

Patent

CA 2736877

Methods for introducing and expressing genes in animal cells are provided comprising infecting the animal cells with live invasive reduced-genome bacteria comprising a eukaryotic expression cassette comprising said gene. Also provided are methods for producing a pluripotent stem (iPS) cell from a mammalian somatic cell comprising infecting the somatic cell with live invasive reduced-genome bacteria comprising one or more eukaryotic expression cassettes comprising at least a gene encoding the transcription factor Oct3/4 and a gene encoding a member of the SRY- related HMG-box (Sox) transcription factor family.

Cette invention concerne des procédés pour introduire et exprimer des gènes dans des cellules animales comprenant l'infection des cellules animales avec une bactérie vivante invasive à génome réduit comprenant une cassette d'expression eucaryote contenant ledit gène. Des procédés pour produire une cellule souche pluripotente (iPS) à partir d'une lignée somatique mammalienne sont également décrits, comprenant l'infection de la lignée somatique avec une bactérie vivante invasive à génome réduit comprenant une ou plusieurs cassettes d'expression eucaryotes contenant au moins un gène codant pour le facteur de transcription Oct3/4 et un gène codant pour un membre de la famille des facteurs de transcription Sox (SRY-related HMG-box).

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