Cleavage of caulobacter produced recombinant fusion proteins

Details Cleavage of caulobacter produced recombinant fusion proteins Cleavage of caulobacter produced recombinant fusion proteins

C - Chemistry, Metallurgy

12

N

C12N 15/62 (2006.01) C07K 1/12 (2006.01) C07K 14/00 (2006.01) C07K 14/08 (2006.01) C07K 14/195 (2006.01) C07K 14/21 (2006.01) C07K 14/245 (2006.01) C07K 19/00 (2006.01) C12N 15/31 (2006.01) C12P 21/00 (2006.01)

Patent

CA 2237704

This invention provides a method of cleaving target proteins from Caulobacter S-layer protein under mild acid conditions whereby a fusion protein secreted by the Caulobacter and comprising the target protein and at least a Caulobacter S-layer secretion signal may be cleaved at a aspartate-proline dipeptide without solubilizing the fusion protein. This method may be carried out while the fusion protein is in an insoluble aggregate which facilitates recovery of the protein. This invention also provides a method of preparing a DNA construct for expression of the fusion protein and a method of preparing the fusion protein.

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