Clinical procedure for measuring lipoprotein cholesterols

Details Clinical procedure for measuring lipoprotein cholesterols Clinical procedure for measuring lipoprotein cholesterols

G - Physics

01

N

150/11, 324/23

G01N 27/26 (2006.01) C12Q 1/60 (2006.01) G01N 27/447 (2006.01) G01N 33/92 (2006.01)

Patent

CA 1096757

ABSTRACT OF THE DISCLOSURE An electrophoresis method of determining the concen- tration of high density lipoprotein (HDL) cholesterol in body fluids and simultaneously determining the concentrations of very low density lipoprotein (VLDL) and low density lipoprotein (LDL) cholesterols in the fluid. The method includes applying a small sample of the fluid to an electrophoresis support med- ium, applying a direct current across the medium, applying a developing substrate to the electrophoresed lipoproteins and quantitatively determining the concentration of each lipo- protein cholesterol. This method does permit direct and simul- taneous measurement of each lipoprotein cholesterol fraction while eliminating precipitation of each fraction as required by the prior art.

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