Clinical sample preparation on a microfluidic platform

G - Physics – 01 – N

Patent

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G01N 1/40 (2006.01) B81B 1/00 (2006.01) C07H 21/00 (2006.01) C12N 15/10 (2006.01)

Patent

CA 2680558

Genotyping clinical samples (e.g. whole blood and buccal swabs) for molecular diagnostics is typically performed in laboratories using specialized equipment by trained operators. The present invention provides for an integrated microfluidic approach wherein cell lysis, molecules of interest cleanup, molecules of interest isolation/purification and molecules of interest transport approach is demonstrated. A magnet is focussed within the microfluidic channel and facilitates the magnetic bead movement which binds the molecules of interest. Further a procedure is developed to indefinitely re-use the chips with no notable degradation in performance.

Le génotypage d'échantillons cliniques (par exemple, sang entier et écouvillons buccaux) pour un diagnostic moléculaire est typiquement effectué dans des laboratoires utilisant un équipement spécialisé par des opérateurs entraînés. La présente invention concerne une approche microfluidique intégrée dans laquelle une approche de lyse cellulaire, de nettoyage de molécules d'intérêt, d'isolation/purification de molécules d'intérêt et de transport de molécules d'intérêt est démontrée. Un aimant est focalisé à l'intérieur du canal microfluidique et facilite le mouvement de bille magnétique qui lie les molécules d'intérêt. L'invention concerne également un mode opératoire qui est développé pour réutiliser de façon indéfinie les puces sans dégradation notable de la performance.

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