Clonal pre-amplification in emulsion

Details Clonal pre-amplification in emulsion Clonal pre-amplification in emulsion

C - Chemistry, Metallurgy

12

P

C12P 19/34 (2006.01) C12N 15/00 (2006.01) C12Q 1/68 (2006.01) C40B 50/06 (2006.01)

Patent

CA 2745258

The present invention is directed to a process for clonal pre-amplification of a nucleic acid comprising the steps of (i) providing a plurality of different nucleic acid molecules (b) attaching adaptor sequences to the 3' ends and 5' ends of said nucleic acid molecules (c) preparing a water in oil emulsion characterized in that the majority of water droplets comprises one or none member of said plurality of different nucleic acid molecules (d) clonally amplifying said plurality of different nucleic acid molecules. In particular, said different nucleic acid molecules are mRNA molecules.

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