C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 5/16 (2006.01)
Patent
CA 2617047
Disclosed herein are a cloned canine and a production method thereof. The method comprises the steps of enucleating the oocyte of a canine to prepare an enucleated recipient oocyte, conducting nuclear transfer into the enucleated oocyte using a canine somatic cell as a nuclear donor cell under optimized conditions so as to prepare a nuclear transfer embryo, and transferring the nuclear transfer embryo into the oviduct of a surrogate mother. The present invention provides a method for producing cloned canines and thus, can contribute to the development of studies in veterinary medicine, anthropology and medical science such as the propagation of superior canines, the conservation of rare or nearly extinct canines, xenotransplantation and disease animal models.
L'invention concerne un canidé cloné et un procédé de production associé. Ledit procédé consiste à énucléer un ovocyte d'un canidé pour préparer un ovocyte récepteur énucléé, à conduire le transfert nucléaire dans l'ovocyte énucléé à l'aide d'une cellule somatique de canidé en tant que cellule donneuse nucléaire dans des conditions optimales de façon à préparer un embryon de transfert nucléaire, et à transférer ledit embryon dans l'oviducte d'une mère porteuse. L'invention concerne un procédé permettant de produire des canidés clonés, ce qui peut contribuer au développement d'études en médecine vétérinaire, de l'anthropologie et des sciences médicales, notamment la propagation de canidés supérieurs, la conservation de races de canidés rares ou presque disparues, la xénotransplantation et les modèles de maladies animales.
Hwang Woo Suk
Jang Goo
Kang Sung Keun
Kim Hye Jin
Kim Joung Ju
Blake Cassels & Graydon Llp
Seoul National University Industry Foundation
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