Cloned cells, embryos, and avians and methods, of producing...

Details Cloned cells, embryos, and avians and methods, of producing... Cloned cells, embryos, and avians and methods, of producing...

C - Chemistry, Metallurgy

12

N

C12N 15/00 (2006.01) A01K 67/027 (2006.01) C07K 14/18 (2006.01) C12N 15/85 (2006.01)

Patent

CA 2421174

Methods for producing cloned non-human animals by nuclear transfer and by combinations of nuclear transfer and ovum transfer, employing near-infrared visualization of the recipient cell nucleus are described herein. Transgenic, knock-out, and knock-in avians are provided. Method for producing eggs which contain exogenous proteins are encompassed by the present invention. Thus, the instant invention satisfies the need for an effective route to the generation of cloned avians.

L'invention concerne des procédés permettant de produire des animaux clonés par transfert nucléaire et par des combinaisons transfert nucléaire et transfert d'ovule, à l'aide d'une visualisation en proche infrarouge du noyau cellulaire récepteur. L'invention concerne également des oiseaux transgéniques, des oiseaux <= knock-out >= et des oiseaux <= knock-in >=. L'invention concerne en outredes procédés permettant de produire des oeufs qui contiennent des protéines exogènes. Cette invention répond ainsi au besoin de définir une méthode efficace permettant la production d'oiseaux clonés.

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