Cloned plant p-hydroxyphenyl pyruvic acid dioxygenase

C - Chemistry – Metallurgy – 12 – N

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C12N 15/53 (2006.01) A01H 5/00 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2245003

A cDNA clone from Arabidopsis thaliana, pHPP1.5, SEQ ID NO:1, which encodes the enzyme p-hydroxyphenyl pyruvic acid dioxygenase, is disclosed. A vector and microbial host containing a DNA sequence coding for the expression of Arabidopsis thaliana p-hydroxyphenyl pyruvic acid dioxygenase, and a genetic construct containing a DNA sequence coding for the expression of Arabidopsis thaliana p-hydroxyphenyl pyruvic acid dioxygenase, together with a promoter located 5' to the DNA coding sequence and a 3' termination sequence, are also disclosed. A method of creating a transgenic plant in which production of plastoquinones, vitamin E, and carotenoids has been modified, is also disclosed.

L'invention concerne un clône d'ADNc d'Arabidopsis thaliana pHPP1.5, SEQ ID NO:1, qui code pour l'enzyme dioxygénase de l'acide p-hydroxyphénylpyruvique. Elle concerne également un vecteur et un hôte microbien contenant une séquence d'ADN codant pour et exprimant la dioxygénase de l'acide p-hydroxyphénylpyruvique d'Arabidopsis thaliana et un produit d'assemblage génétique contenant une séquence d'ADN codant pour et exprimant la dioxygénase de l'acide p-hydroxyphénylpyruvique d'Arabidopsis thaliana, un promoteur en 5' de la séquence d'ADN codante et une séquence de terminaison en 3'. L'invention concerne aussi un procédé pour créer une plante transgénique dans laquelle la production de plastoquinones, de vitamine E et de caroténoïdes a été modifiée.

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