C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/55 (2006.01) C12N 1/21 (2006.01) C12N 9/10 (2006.01) C12N 9/22 (2006.01) C12N 15/54 (2006.01)
Patent
CA 2084063
2084063 9118916 PCTABS00008 The present invention is directed to recombinant hosts which contain and express various Type II restriction endonuclease and/or modification methylase genes. In particular, the present invention is concerned with the cloned restriction endonucleases, NgoAIII and NgoAI, which recognize and cleave within or near the double-stranded DNA sequence, 5' CCGCGG 3' and 5' PuGCGCPy 3', respectively. Also provided in this invention are cloned modification methylase genes corresponding to said restriction endonucleases. This invention is further concerned with a cloned modification methylase, M.NgoAII. One source of theses enzymes is Neisseria gonorrhoeae, although other microorganisms may be used to isolate the restriction endonuclease isoschizomers and modification methylase isoschizomers of this invention.
Chatterjee Deb K.
Hammond Alan W.
Chatterjee Deb K.
Gowling Lafleur Henderson Llp
Hammond Alan W.
Life Technologies Inc.
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