Cloning and expression of a gene encoding bryodin 1 from...

Details Cloning and expression of a gene encoding bryodin 1 from... Cloning and expression of a gene encoding bryodin 1 from...

C - Chemistry, Metallurgy

12

N

C12N 15/56 (2006.01) A61K 47/48 (2006.01) C07K 14/415 (2006.01) C12N 15/29 (2006.01) C12N 15/62 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2148724

The molecular cloning and expression of biologically active ribosome-inactivating protein bryodin 1 are described. A complete amino acid and oligonucleotide sequence encoding bryodin 1 are also described. Further, plasmids, expression vectors comprising a nucleotide sequence encoding bryodin 1 and transformed host cells are described. Isolation and characterization of the nucleotide sequence for bryodin 1 enables the recombinant production of large amount of bryodin 1 for use in vitro or in vivo directly or as ligand/toxin conjugates or fusion proteins. These compositions can be used to selectively kill undesired cells such as cancer cells, infected cells, bacteria and the like.

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