C - Chemistry – Metallurgy – 12 – P
Patent
C - Chemistry, Metallurgy
12
P
C12P 21/02 (2006.01) C07K 14/31 (2006.01) C12N 9/10 (2006.01) C12N 9/14 (2006.01) C12N 9/24 (2006.01) C12N 9/40 (2006.01) C12N 15/55 (2006.01) A61K 38/00 (2006.01)
Patent
CA 2150555
The present invention involves the production of large quantities of human alpha-Gal A by cloning and expressing the alpha-Gal A coding sequence in eukaryotic host cell expression systems. The eukaryotic expression systems, and in particular the mammalian host cell expression system described herein provide for the appropriate cotranslational and posttranslational modifications required for proper processing, e.g., glycosylation, phosphorylation, etc. and sorting of the expression product so that an active enzyme is produced. In addition, the expression of fusion proteins which simplify purification is described. Using the methods described herein, the recombinant alpha-Gal A is secreted by the engineered host cells so that it is recovered from the culture medium in good yield. The alpha-Gal A produced in accordance with the invention may be used, but is not limited to, in the treatment in Fabry Disease; for the hydrolysis of alpha-galactosyl residues in glycoconjugates; and/or for the conversion of the blood group B antigen on erythrocytes to the blood group 0 antigen.
Bishop David F.
Desnick Robert J.
Ioannou Yiannis A.
Mount Sinai School Of Medicine
Osler Hoskin & Harcourt Llp
The Mount Sinai School Of Medicine Of The City University Of New
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