Cloning and expression of gonadotropin-releasing hormone...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/16 (2006.01) A61K 39/00 (2006.01) C07H 21/04 (2006.01) C07K 14/72 (2006.01) C07K 16/26 (2006.01) C12N 5/10 (2006.01) C12N 15/11 (2006.01) C12N 15/62 (2006.01) C12P 21/08 (2006.01) G01N 33/76 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2138999

The present invention relates to the GnRH-R genes and proteins. The DNA sequences disclosed herein may be engineered into expression systems designed for the production of GnRH-R and/or cell lines which express the GnRH-R and preferably respond to GnRH induced signal transduction. Such cell lines may advantageously be used for screening and identifying GnRH agonists and antagonists. In accordance with another aspect of the invention, the GnRH DNA, antisense oligonucleotide sequences, the GnRH expression products, and antibodies to such products may be used in the diagnosis and therapy of reproductive disorders associated with abnormal expression of the GnRH-R; e.g., overexpression, underexpression or expression or expression of a dysfunctional mutant receptor. Transgenic animals containing the GnRHR transgene may be used as animal models for the evaluation of GnRH analogs in vivo.

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