Cloning and expression of protein antigen of toxoplasma gondii

Details Cloning and expression of protein antigen of toxoplasma gondii Cloning and expression of protein antigen of toxoplasma gondii

C - Chemistry, Metallurgy

12

N

C12N 15/30 (2006.01) A61K 39/002 (2006.01) A61K 39/395 (2006.01) A61K 47/48 (2006.01) C07H 21/00 (2006.01) C07K 14/45 (2006.01) C07K 16/20 (2006.01) C12P 21/08 (2006.01) G01N 33/566 (2006.01) G01N 33/569 (2006.01) G01N 33/577 (2006.01) A61K 39/00 (2006.01)

Patent

CA 2098845

2098845 9211366 PCTABS00014 A purified antigen of T. gondii having a molecular weight of 54-kDa (as determined by Western blotting) is provided which is reactive with antibodies raised against a recombinant protein obtained by expression of a defined T. gondii cDNA (Figure 2) in a host cell; also provided are immunogenic fragments of the 54-kDa antigen. Recombinant DNA encoding the 54-kDa antigen, host cells for expression of such DNA, a vaccine for treating or preventing toxoplasmosis infection in humans and animals, and test kits for detection of T. gondii antigen or antibody are also described.

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