Cloning and expression of recombinant adhesive protein...

Details Cloning and expression of recombinant adhesive protein... Cloning and expression of recombinant adhesive protein...

C - Chemistry, Metallurgy

12

P

C12P 21/06 (2006.01) C12N 1/20 (2006.01)

Patent

CA 2575353

The present invention includes a Mytilus edulis cDNA having a nucleotide sequence that encodes for the Mytilus edulis foot protein-2 (Mefo-2), an example of mollusk foot protein. Mefp-2 is an integral component of the blue mussels' adhesive protein complex, which allows the mussel to attach to objects underwater. The isolation, purification and sequencing of the Mefp-2 gene will allow researchers to produce Mefp-2 protein using genetic engineering techniques. The discovery of Mefp-2 gene sequences will also allow scientists to better understand how the blue mussel creates its waterproof adhesive protein complex.

La présente invention concerne un ADN complémentaire de Mytilus edulis comprenant une séquence nucléotidique codant pour la protéine de pied de Mytilus edulis de type 2 (Mefp-2), un type de protéine de pied de mollusque. Mefp-2 fait partie intégrante d'un complexe de protéines adhésives de la moule bleue qui permet à la moule de se fixer à des objets sous l'eau. L'isolement, la purification et le séquençage du gène Mefp-2 permettra aux chercheurs de produire une protéine Mefp-2 au moyen de techniques de génie génétique. La découverte de séquences de gènes Mefp-2 permettra également aux scientifiques de mieux comprendre comment la moule bleue crée son complexe de protéines adhésives résistant à l'eau.

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