Cloning and expression of recombinant adhesive protein...

C - Chemistry – Metallurgy – 07 – K

Patent

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C07K 14/435 (2006.01) C12N 15/12 (2006.01)

Patent

CA 2575368

The present invention comprises a Mytilus edulis cDNA sequence having a nucleotide sequence that encodes for the Mytilus edulis foot protein-1 (Mefp- 1), an example of a mollusk foot protein. Mefp-1 is an integral component of the blue mussels' adhesive protein complex, which allows the mussel to attach to objects underwater. The isolation, purification and sequencing of the Mefp- 1 gene will allow researchers to produce Mefp-1 protein using genetic engineering techniques. The discovery of Mefp-1 gene sequence will also allow scientists to better understand how the blue mussel creates its waterproof adhesive protein complex.

L'invention concerne une séquence d'ADNc de Mytilus edulis possédant une séquence de nucléotides codant pour la protéine-1 du pied de Mytilus edulis (Mefp-1), en tant qu'exemple de protéine du pied du mollusque. Mefp-1 est un élément solidaire du complexe de protéines adhésives de la moule bleue, ce qui permet à la moule de se fixer à des objets sous l'eau. L'isolation, la purification et le séquençage du gène Mefp-1 permettra à la recherche de produire la protéine Mefp-1 au moyen de techniques de génie génétique. La découverte de la séquence génique de Mefp-1 permettra également à la science de mieux comprendre la façon dont la moule bleue créée son complexe de protéines adhésives étanche à l'eau.

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