Cloning and expression of the chondroitinase i and ii genes...

Details Cloning and expression of the chondroitinase i and ii genes... Cloning and expression of the chondroitinase i and ii genes...

C - Chemistry, Metallurgy

12

N

C12N 15/60 (2006.01) C12N 1/21 (2006.01) C12N 9/88 (2006.01)

Patent

CA 2161125

This invention relates to the DNA sequence encoding the major protein component of chondroitinase ABC, which is referred to as "chondroitinase I", from Proteus vulgaris (P. vulgaris), which is contained in the Nsi fragment shown in the figure. This invention further relates to the DNA sequence encoding a second protein component of chondroitinase ABC, which is referred to as "chondroitinase II", from P. vulgaris, to the cloning and expression of the genes containing these DNA sequences, to the amino acid sequences of the recombinant chondroitinase I and II, and to methods for the isolation and purification of recombinant chondroitinase I or II. These methods provide significantly higher yields and purity than those obtained by adapting for the recombinant enzymes the method previously used for isolating and purifying native chondroitinase I enzyme from P. vulgaris.

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