Cloning and expression of transforming growth factor beta-2

Details Cloning and expression of transforming growth factor beta-2 Cloning and expression of transforming growth factor beta-2

C - Chemistry, Metallurgy

12

N

530/13, 195/1.23

C12N 15/18 (2006.01) A61K 38/18 (2006.01) C07K 14/495 (2006.01) C12N 5/00 (2006.01) C12N 5/10 (2006.01) C12N 15/12 (2006.01) C12N 15/85 (2006.01) C12N 15/86 (2006.01) C12P 21/02 (2006.01)

Patent

CA 2005459

ABSTRACT cDNA clones coding for TGF-.beta.2 which are used to construct expression vectors capable of directing the high- level expression of mature, biologically active TGF-.beta.2, as well as precursor TGF-.beta.2 forms, in transfected Chinese Hamster Ovary cells (CHO cells) and transfected COS cells are described. CHO and COS transfectants secreting TGF-.beta.2 at high levels are also described. CHO cells transfected with a plasmid vector carrying the complete 414 amino acid simian TGF-.beta.2 precursor secrete approximately 5 µg per ml culture media.

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