Cloning and recombinant production of receptor(s) of the...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/54 (2006.01) A61K 38/18 (2006.01) A61K 38/45 (2006.01) A61K 39/395 (2006.01) C07H 21/04 (2006.01) C07K 14/495 (2006.01) C07K 14/71 (2006.01) C07K 16/22 (2006.01) C12N 9/12 (2006.01) C12N 15/18 (2006.01) C12P 21/08 (2006.01) C12Q 1/68 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2086327

2086327 9220793 PCTABS00017 In accordance with the present invention, there are provided novel receptor proteins characterized by having the following domains, reading from the N-terminal end of said protein: an extracellular, ligand-binding domain, a hydrophobic, trans-membrane domain, and an intracellular, receptor domain having serine kinase-like activity. The invention receptors optionally further comprise a second hydrophobic domain at the amino terminus thereof. The invention receptor proteins are further characterized by having sufficient binding affinity for at least one member of the activin/TGF-.beta. superfamily of polypeptide growth factors such that concentrations of Í 10 nM of said polypeptide growth factor occupy ã 50 % of the binding sites of said receptor protein. A presently preferred member of the invention superfamily of receptors binds specifically to activins, in preference to inhibins, transforming growth factor-.beta., and other non-activin-like proteins. DNA sequences encoding such receptors, assays employing same, as well as antibodies derived therefrom, are also disclosed.

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