Cloning and sequencing of pyruvate decarboxylase (pdc) genes...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/60 (2006.01) C07K 16/40 (2006.01) C12N 9/88 (2006.01) C12N 15/31 (2006.01) C12N 15/63 (2006.01) C12P 7/06 (2006.01) C12P 21/00 (2006.01) C12Q 1/527 (2006.01) C12Q 1/68 (2006.01) G01N 33/53 (2006.01)

Patent

CA 2445205

The invention provides isolated nucleic acids molecules which encode pyruvate decarboxylase enzymes having improved decarboxylase activity, substrate affinity, thermostability, and activity at different pH. The nucleic acids of the invention also have a codon usage which allows for high expression in a variety of host cells. Accordingly, the invention provides recombinant expression vectors containing such nucleic acid molecules, recombinant host cells comprising the expression vectors, host cells further comprising other ethanologenix enzymes, and methods for producing useful substances, e.g., acetaldehyde and ethanol, using such host cells.

La présente invention concerne des molécules d'acides nucléiques isolées qui codent des enzymes pyruvate décarboxylase présentant une activité décarboxylase, une affinité pour le substrat, une thermostabilité et une activité améliorées à différents pH. Les acides nucléiques selon l'invention présentent également une utilisation du codon qui assure une forte expression dans une variété de cellules hôtes. Par conséquent, la présente invention concerne des vecteurs d'expression de recombinaison contenant de telles molécules d'acides nucléiques, des cellules hôtes de recombinaison comprenant les vecteurs d'expression, des cellules hôtes comprenant d'autres enzymes éthanologènes ainsi que des procédés de production de substances utiles, par exemple, de l'acétaldéhyde et de l'éthanol, à l'aide de telles cellules hôtes.

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