Cloning and sequencing of the dna fragment which codes for...

C - Chemistry – Metallurgy – 12 – N

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530/13, 167/137,

C12N 15/31 (2006.01) A61K 39/10 (2006.01) C07K 14/235 (2006.01) C12Q 1/68 (2006.01) A61K 39/00 (2006.01)

Patent

CA 1340373

The pertussis toxin or one or more subunits thereof are useful for the preparation of vaccines and diagnostic kits. Obtainment of the toxin may be achieved by cloning and sequencing the Eco RI fragment of B. pertussis chromosomal DNA with 4696 base pairs, i.e. the fragment containing the genes which code for the five subunits of the pertussis toxin. A hybrid plasmid containing this DNA fragment or its further fragments and a micro-organism transformed by the hybrid plasmid are capable of expressing the cloned DNA fragment or further fragments thereof by synthesis of the pertussis toxin or one or more subunits thereof.

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