Cloning by complementation and related processes

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) C07K 14/435 (2006.01) C07K 14/82 (2006.01) C12N 9/16 (2006.01) C12N 15/10 (2006.01) C12N 15/12 (2006.01) C12N 15/55 (2006.01) C12N 15/81 (2006.01)

Patent

CA 2080920

Disclosed are methods for detecting mammalian genes encoding proteins which can function in microorganisms, particu- larly yeast, to modify, complement, or suppress a genetic defect associated with an identifiable phenotypic alteration or character- istic in the microorganism. Disclosed also are mamalian DNA sequences cloned bythe above method, as well as polypeptide pro- ducts of the expression of the DNA sequences in procaryotic or eucaryotic host cells and antibody substances which are specifically immunoreactive with said expression products. More specifically, the present invention methods for cloning mam- malian genes which encode products which modify, complement or suppress a genetic defect in a biochemical pathway in which cAMP participates or in a biochemical pathway which is controlled, directly or indirectly, by an RAS protein, to products (RNA, proteins) encoded by the mammalian genes cloned in this manner, and to antibodies which can bind the encoded proteins.

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