Cloning cartridges and expression vectors in gram-negative...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/74 (2006.01) C12N 1/21 (2006.01) C12N 9/02 (2006.01) C12N 15/11 (2006.01) C12N 15/70 (2006.01) C12N 15/73 (2006.01) C12N 15/78 (2006.01)

Patent

CA 2069741

Cloning cartridges comprising a positive regulatory gene nahR from plasmid NAH7, a promoter Pg that is regulated by nahR, a multiple cloning site, a transcription terminator, and a gene conferring tetracycline resistance. Such cartridges can be in- serted into plasmids of choice to form novel expression vectors in which high level gene expression is inducible with an inexpen- sive non-toxic inducer at low concentrations.

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