Cloning, expression and sequencing of an ester hydrolase...

Details Cloning, expression and sequencing of an ester hydrolase... Cloning, expression and sequencing of an ester hydrolase...

C - Chemistry, Metallurgy

12

N

195/131, 195/39,

C12N 15/72 (2006.01) C12N 9/16 (2006.01) C12N 9/18 (2006.01) C12N 15/55 (2006.01) C12P 7/40 (2006.01) C12P 7/42 (2006.01) C12P 41/00 (2006.01)

Patent

CA 2023892

ABSTRACT OF THE DISCLOSURE The present invention is directed to the production of an ester hydrolase, in particular, utilizing recombinant molecules containing an ester hydrolase gene from Pseudomonas fluorescens and the generation of a cloned hydrolase and mutants thereof in Escherichia coli. The cloned hydrolases so produced are especially useful in a process in which R-,5-naproxen esters are enzymatically hydrolyzed in an enentiospecific manner.

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