Cloning method by multiple digestion, vectors for...

C - Chemistry – Metallurgy – 12 – N

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Details

C12N 15/10 (2006.01) C12N 1/21 (2006.01) C12N 9/22 (2006.01) C12N 15/70 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2312029

The invention concerns a novel cloning method based on the systematic, combined and preparatory use of restriction sites present on the inserts constituting a complementary or genome DNA bank. The invention also concerns the applications of said method and the resulting banks.

La présente invention concerne une nouvelle méthode de clonage fondée sur l'utilisation systématique, combinée, et préparative des sites de restriction présents sur les inserts constituant une banque d'AND complémentaire ou génomique. L'invention concerne également les applications de ce procédé ainsi que les vecteurs pour sa mise en oeuvre et les banques obtenues.

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