Cloning of the gene coding the isoamylase enzyme and its use...

Details Cloning of the gene coding the isoamylase enzyme and its use... Cloning of the gene coding the isoamylase enzyme and its use...

C - Chemistry, Metallurgy

12

N

195/1.2, 195/1.2

C12N 1/20 (2006.01) C12N 1/21 (2006.01) C12N 9/44 (2006.01) C12N 15/56 (2006.01) C12N 15/70 (2006.01)

Patent

CA 1308373

CLONING OF THE GENE CODING THE ISOAMYLASE ENZYME AND ITS USE IN THE PRODUCTION OF SAID ENZYME OR THE LIKE Abstract of the Disclosure A fragment of DNA obtained from a bacterium of the genus Pseudomonas containing the coding gene for the isoamylase enzyme is novel. Also novel are replicable recombinant DNA molecules for expression in host micro- organisms containing the aforementioned DNA fragment or coding regions thereof for mature isoamylase or for a polypeptide having biological activity equivalent to that of isoamylase. Also novel are host micro-organisms trans- formed by the aforementioned molecules of recombinant DNA capable of expressing and/or secreting the mature isoamylase enzyme or a polypeptide having a biological activity equivalent to that of isoamylase. The resulting isoamylase is specified in terms of amino acid sequence, structure and biological activity.

573417

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