Cloning pigs using donor nuclei from differentiated cells

A - Human Necessities – 01 – K

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A01K 67/027 (2006.01) A61K 35/12 (2006.01) A61K 48/00 (2006.01) A61K 49/00 (2006.01) C12N 5/00 (2006.01) C12N 5/10 (2006.01) C12N 15/00 (2006.01) C12N 15/87 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2295294

An improved method of nuclear transfer involving the transplantation of donor differentiated pig cell nuclei into enucleated pig oocytes is provided. The resultant nuclear transfer units are useful for multiplication of genotypes and transgenic genotypes by the production of fetuses and offspring. Production of genetically engineered or transgenic pig embryos, fetuses and offspring is facilitated by the present method since the differentiated cell source of the donor nuclei can be genetically modified and clonally propagated.

La présente invention concerne un procédé amélioré de transfert nucléaire impliquant la transplantation de noyaux de cellules donneuses différenciées de porc dans des ovocytes de porc énucléés. Les unités de transfert nucléaire résultantes sont utilisées pour la multiplication de génotypes et de génotypes transgéniques par la production de foetus et de progéniture. La production d'embryons, de foetus et de progéniture de porcs transgéniques ou mis au point par génie génétique est facilitée par le présent procédé du fait que la source de cellules différenciées de noyaux donneurs peut être génétiquement modifiée et propagée par clonage.

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