Cloning system for kluyveromyces species

Details Cloning system for kluyveromyces species Cloning system for kluyveromyces species

C - Chemistry, Metallurgy

12

N

195/1.18, 195/1.

C12N 1/16 (2006.01) C07K 14/43 (2006.01) C12N 9/34 (2006.01) C12N 9/64 (2006.01) C12N 15/00 (2006.01) C12N 15/68 (2006.01) C12N 15/81 (2006.01) C12N 15/90 (2006.01) C12P 21/02 (2006.01)

Patent

CA 1224735

CLONING SYSTEM FOR KLUYVEROMYCES SPECIES Abstract of the disclosure A new cloning system is described capable of expressing genetic material derived from recombinant DNA material, which comprises a yeast of the genus Kluyveromyces as a host. Suitable vectors are e.g. vectors containing autonom- ously replicating sequences (ARS) and vectors containing homologous Kluyveromyces DNA acting as a site for recombin- ation with the host chromosome. New and preferred vectors are those containing ARS sequences originating from Kluyvero- myces (KARS vectors). The genetically engineered new strains of Kluyveromyces produce, inter alia, lactase and chymosin.

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